![]() The optimal numbers and stabilities of reference genes varied between the two temperature treatments (27☌ and 4☌). Data were analyzed using the ΔCt method, BestKeeper, NormFinder, and geNorm. ![]() Specifically, the performance and stabilities of these genes were compared in adult wasps maintained in a growth condition at 27☌ (normal storage conditions) and in adults obtained from pupae refrigerated at 4☌ for five days (cold storage conditions). In this study, the expression profiles of eight candidate housekeeping genes, 18S ribosomal ( 18S rRNA), elongation factor ( EF1), glyceraldehyde-3-phosphate dehydrogenase ( GAPDH), ribosomal protein L10 ( RPL10), ribosomal protein L17 ( RPL17), histone 3 ( H3), arginine kinase ( AK), amd β-Actin ( ACTB), were evaluated in the parasitic wasp Cotesia chilonis in response to different temperatures. ![]() ![]() In quantitative real-time PCR (qRT-PCR), data are normalized using reference genes, which helps to control for internal differences and reduce error among samples.
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